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The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min
Tumor Biol 2013;34(6):3913-3922
Since Glutathione helps slow down melanin synthesis and supports skin cell renewal, it can gradually lighten the darker pigmentation caused by sun exposure
Bioinformatics analysis, including hierarchical cluster, gene ontology, pathway, and network, was performed using Blast2GO (Version 3.5.0), the online Kyoto Encyclopedia of Genes and Genomes (KEGG) database ( and the InAct molecular interaction database ( Differentially expressed proteins between the two groups were screened using fold change method (2 fold)
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